The ubiquitin-activating enzyme E1-like protein in lung cancer cell lines
نویسندگان
چکیده
منابع مشابه
Evolution of the Ubiquitin-Activating Enzyme Uba1 (E1)
Ubiquitin tags diseased proteins and initiates an enzyme conjugation cascade, which has three stages. The first-stage enzyme Uba1 (E1) has evolved only modestly from slime mold to humans, and is > 14 times larger than Ub. Here we use critical point thermodynamic scaling theory to connect Uba1 (E1) evolution from yeast and slime mold to fruit flies and humans to subtle changes in its amino acid ...
متن کاملMathematical model for the ubiquitin activating enzyme E1
The ubiquitin-activating enzyme E1 (EC 6.3.2.19) represents the first step in the degradation of proteins by the ubiquitin proteasome pathway. E1 transfers ubiquitin from the ubiquitinated E1 to the ubiquitin carrier proteins (E2), ubiquitin-protein ligases (E3) and proteins. This process is rather complex, and known from the work of Haas, Ciechanover, Hershko, Rose and others. The occurrence o...
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Protein ubiquitination plays an important role in the regulation of almost every aspect of eukaryotic cellular function; therefore, its destabilization is often observed in most human diseases and cancers. Consequently, developing inhibitors of the ubiquitination system for the treatment of cancer has been a recent area of interest. Currently, only a few classes of compounds have been discovere...
متن کاملHomology Modelling of Human E1 Ubiquitin Activating Enzyme.
Human E1 is a key player in protein ubiquitination, however the E1 structure is not available. In this paper, we describe the derivation of a human E1 structure using molecular modelling based on the crystal structure of S. cerevisiae E1 and M. Musculus E1. Key interactions between our E1 model and ubiquitin are also discussed.
متن کاملUbiquitin-activating enzyme, E1, is phosphorylated in mammalian cells by the protein kinase Cdc2.
The ubiquitin-activating enzyme (E1) is the first enzyme in the pathway leading to formation of ubiquitin-protein conjugates. E1 was found to be phosphorylated in cells of a mouse mammary carcinoma cell line, FM3A. Peptide mapping of trypsin digests of labeled E1 indicated that two oligopeptides were mainly phosphorylated in vivo. The same oligopeptides were also labeled in vitro on Cdc2 kinase...
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ژورنال
عنوان ژورنال: International Journal of Cancer
سال: 2000
ISSN: 0020-7136,1097-0215
DOI: 10.1002/(sici)1097-0215(20000315)85:6<871::aid-ijc22>3.0.co;2-o